#2.1.1a
the use of microscopy to observe and investigate different types of cell and cell structure in a range of eukaryotic organisms
To include an appreciation of the images produced by a range of microscopes; light microscope, transmission electron microscope, scanning electron microscope and laser scanning confocal microscope.
#2.1.1b
the preparation and examination of microscope slides for use in light microscopy
Including the use of an eye piece graticule and stage micrometer.
PAG 1
#2.1.1c
the use of staining in light microscopy
To include the use of differential staining to identify different cellular components and cell types.
PAG 1
#2.1.1d
the representation of cell structure as seen under the light microscope using drawings and annotated diagrams of whole cells or cells in sections of tissue
#2.1.1e
the use and manipulation of the magnification formula
\(\text{magnification} = \dfrac{\text{image size}}{\text{object size}}\)
#2.1.1f
the difference between magnification and resolution
To include an appreciation of the differences in resolution and magnification that can be achieved by a light microscope, a transmission electron microscope and a scanning electron microscope.
#2.1.1g
the ultrastructure of eukaryotic cells and the functions of the different cellular components
To include the following cellular components and an outline of their functions: nucleus, nucleolus, nuclear envelope, rough and smooth endoplasmic reticulum (ER), Golgi apparatus, ribosomes, mitochondria, lysosomes, chloroplasts, plasma membrane, centrioles, cell wall, flagella and cilia.
#2.1.1h
photomicrographs of cellular components in a range of eukaryotic cells
To include interpretation of transmission and scanning electron microscope images.
#2.1.1i
the interrelationship between the organelles involved in the production and secretion of proteins
No detail of protein synthesis is required.
#2.1.1j
the importance of the cytoskeleton
To include providing mechanical strength to cells, aiding transport within cells and enabling cell movement.
#2.1.1k
the similarities and differences in the structure and ultrastructure of prokaryotic and eukaryotic cells.
PAG 1