GCSE Biology Specification

OCR B J257

Section B2.4: How can we identify the cause of an infection?

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#B2.4.1

a) describe ways in which diseases, including plant diseases, can be detected and identified, in the lab and in the field

b) describe how to use a light microscope to observe microorganisms
PAG1

#B2.4.2

describe and explain the aseptic techniques used in culturing organisms
PAG7

#B2.4.3

calculate cross-sectional areas of bacterial cultures and of clear zones around antibiotic discs on agar jelly using \(πr^2\)
PAG7

#B2.4.4

describe how monoclonal antibodies are produced

including the following steps:
- antigen injected into an animal
- antibody-producing cells taken from animal
- cells producing the correct antibody selected then cultured

#B2.4.5

describe some of the ways in which monoclonal antibodies can be used in diagnostic tests